Keyword: Microscopy

ABSTRACT: Recent advances in artificial intelligence—particularly Vision–Language Models (VLMs)—offer promising avenues for enhancing microscopic diagnostics. This review synthesizes the current landscape of VLM applications across microbiology, hematology, cytology, and histopathology, spanning tasks such as Gram stain classification, cell-type recognition, feature localization, captioning, and report drafting. We outline how VLMs integrate visual features with domain-specific prompts to support triage, decision support, and quality control, while highlighting opportunities for few-shot and zero-shot generalization to rare findings. In parallel, we compare conventional convolutional pipelines with VLM-enhanced workflows, emphasizing gains in scalability, reproducibility, and explainability through multimodal rationales and grounded visual evidence. Key challenges include data curation and harmonization across laboratories, domain shift from variable staining and optics, bias and safety risks, limited task-relevant benchmarks, and the need for rigorous human-in-the-loop evaluation in clinical contexts. We propose a practical roadmap for deployment—covering dataset governance, prompt and template standardization, uncertainty reporting, and audit trails—alongside research priorities in robust evaluation, privacy-preserving learning, and alignment with clinical guidelines. Overall, VLMs are poised to complement expert microscopy by accelerating routine workflows and improving documentation, provided their adoption is guided by transparent validation and fit-for-purpose governance.